Annex D-2.2: Instructions for completion of Shiga-toxin-producing Escherichia coli (STEC) in beef trimmings verification sampling template
Color coding of the template
Peach cells: Data needs to be entered by the lab.
Yellow cells: Drop-down menus provide the data options to be entered by the lab.
Blue cells: The data auto populates and is dependent on information entered in other cells.
Enter the laboratory name and city information at the top of the template using the following format: Laboratory name, city.
Completion and verification of sample information
- Enter unique job/project number as found on record/certificate of analysis. This number is issued by the lab for internal tracking purposes.
- Verify the lab name, city (column C) and enter the province (column D).
- Select the appropriate registered establishment number, of the meat establishment for whom the samples have been analyzed, from the drop-down menu. The establishment name and province will auto populate based on the registered establishment number entered.
- Verify the establishment name and province. If this information is not correct, re-check the registered establishment number that was entered. If the establishment you are searching is not available please contact CFIA at email@example.com.
- Enter the unique sample ID as assigned by the laboratory for tracking purposes.
- Enter the product name and a brief description of the product which was analyzed.
- Select the appropriate temperature range from the drop-down menu which encompasses the temperature of the product upon reception at the lab.
- Enter the following dates: the date sampled, date received at lab, and date analysis was completed.
- From the drop-down menu select the screening method used.
- From the drop-down menu, identify the analytical result of the screening method. If a presumptive positive is being reported as a confirmed positive, and no confirmatory method is being performed, go directly to Step 2m.
- If the screening method was positive, use the drop-down menu to identify the confirmatory method used.
- Identify the analytical result of the confirmatory method from the drop-down menu.
- Identify which E. coli serotype was identified, using the drop-down menu. If more than one serotype is identified separate lines of data entry are required for each positive. If a presumptive positive is being reported as a confirmed positive, select presumptive positive from the menu.
If, for whatever reason, the laboratory is unable to analyse the sample submitted for analysis, the operator is expected to submit a replacement sample as soon as possible.
- All results must be reported to the operator as soon as they are available.
- Results entered into the template along with the supporting record/certificate of analysis must be reported to the CFIA through the following dedicated e-mail account: firstname.lastname@example.org.
- The subject line of the e-mail should be of the format: Lab name – Job number.
- E-mails reporting positive (present) results should be classified as high priority or urgent, and submitted within 1 business day.
- Negative (absent) results may be combined into one template and submitted within the last week of each month.
- Please do not report confirmation of sample receipt through this e-mail account.
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