Sampling procedures

Although the Safe Food for Canadians Regulations (SFCR) came into force on January 15, 2019, certain requirements are being phased in over the following 12 to 30 months. For more information, refer to the SFCR timelines.


Sampling is the process of collecting and testing food, ingredients, the environment or other materials. Sampling is commonly used to monitor or verify the effectiveness of control measures put in place to prevent, eliminate or reduce to an acceptable level the hazards that present a risk of contamination to a food. Sampling can also provide assurance that incoming materials, finished products and water meet food safety standards.

It is important to collect food samples that are representative of a lot or a food contact surface being assessed. It is also important to ensure that samples are not compromised when being collected, stored or shipped, as this could lead to inaccurate results.


The Canadian Food Inspection Agency (CFIA) created this document as guidance to help food businesses comply with the Safe Food for Canadians Regulations.

It's your choice!

You may use other guidance developed by provincial governments, industry associations, international partners or academic bodies as long as they can achieve the outcomes identified in the regulations. Always ensure that the guidance you choose is relevant for your particular business, product or products, and market requirements

What is a sample?

A collection of one or more units from a lot drawn for examination or testing purposes.

  • food sample units could be entire packages of food, portions of packaged food, or portions of food being prepared.

What is a random sample?

A sample in which all the units were selected randomly by chance, such that each unit in the lot had the same probability of being chosen at any stage during the sampling process.

What's included

This document provides information on the sampling process, including:

  1. Why sample
  2. What to sample
  3. What sampling plan to use
  4. Where to sample
  5. How to sample
  6. Storing and shipping samples
  7. Testing the samples
  8. What to do with sample results

Refer to the Tell me more! section for additional sources of information that may help you establish sampling procedures.

What's not included

While this document provides general guidance on sampling, it is not exhaustive – the reason for sampling and where the samples are taken will be unique for each business.

Roles and responsibilities

Food businesses are responsible for complying with the law. They demonstrate compliance by ensuring that the commodities and processes for which they are responsible meet regulatory requirements. If a written preventive control plan (PCP) is required, the food business develops a PCP with supporting documents, monitors and maintains evidence of its implementation, and verifies that all control measures are effective.

The CFIA verifies the compliance of a food business by conducting activities that include inspection, and surveillance. When non-compliance is identified, the CFIA takes appropriate compliance and enforcement actions.

Sampling procedures

1. Why sample

Sampling is commonly used to:

  • assess the acceptance of consignments
  • test for batch release
  • control raw materials
  • control in process products
  • assess the finished product
  • monitor, verify and validate the effectiveness of control measures, for example:
    • determine whether control measures such as sanitation procedures are effective in preventing the contamination of a food
    • determine whether the water used in your establishment meets potable water requirements
    • monitor the pH or Aw level of a food at a critical control point to ensure that it is within the critical limits
    • validate the shelf life established for the food
    • assess the acceptability of an ingredient

Sampling is used to assess the presence and level of the following three types of hazards.

Biological hazards

  • Pathogens such as:
    • Listeria, E. coli, Salmonella that are pathogens
  • Micro-organisms that are indicators of unsanitary conditions such as:
    • coliforms and generic E. coli

Chemical hazards

  • Allergens such as peanuts, soy, eggs, milk, tree nuts, wheat, sesame, seafood, mustard, sulphites
  • Drug residues such as antibiotics
  • Pesticide residues such as Dichlorodiphenyltrichloroethane (DDT)
  • Toxins such as aflatoxins in peanuts, Paralytic shellfish poisoning (PSP) and domoic acid in shellfish

Physical hazards

  • Extraneous materials such as glass or metal fragments
  • Bone fragments

2. What to sample

The types of processes, treatment and food prepared in your establishment and the control measures applied will help determine the samples you should take.

The following are common types of samples taken in food production:

  • Environment
  • Finished food
  • Food in-line
  • Water
  • Incoming ingredients


The surfaces in the area or environment where food is prepared are sampled to verify the effectiveness of cleaning and sanitation procedures. For example, samples of food contact surfaces are taken to test for:

  • Adenosine triphosphate (ATP) bioluminescent, an indicator of remaining food debris after cleaning.
  • Remaining viable bacteria such as aerobic colony count (ACC) or coliforms (after sanitizers are applied)
  • Listeria spp. or L. monocytogenes to determine whether control measures put in place are effective

Finished food

The food is sampled at the end of the production line, when it is packaged in its finished form, to verify that it meets specifications and is not contaminated.

Food in-line (during preparation)

Sampling food during preparation can help you assess its characteristics at a specific point in the process. It can also help you monitor critical limits to ensure they are met.


Water is commonly sampled to:

  • assess the safety of the source water in the establishment
  • verify the effectiveness of in-house water treatments such as UV, ozone and chlorine
  • verify that the frequency at which recirculated water, used for processing and washing, is changed and the concentration of sanitizers used are adequate

For more information see the guidance document Water for use in the preparation of food.

Incoming ingredients

Sampling incoming ingredients helps assess a supplier's food safety control measures.

3. What sampling plan to use

The sampling plan you select will depend on the reason for sampling. Statistically based sampling plans should be used when assessing final products. You can find information on sampling plans in the General guidelines on sampling.

Information on sampling plans for microbiological analysis is also available in the document Microorganisms in foods 2: Sampling for microbiological analysis: Principles and specific applications published by the International Commission on Microbiological Specifications for Food.

4. Where to sample

Where to sample depends on what you are sampling and the reason you are sampling. The following provides examples of where different types of samples are taken based on what is being assessed.

Environmental samples

The sites for collecting environmental samples are selected according to what information you are trying to gather. For example, your goal may be to assess the effectiveness of your sanitation procedures in an establishment; or your goal may be to monitor the microbiological state of the environment while food is being prepared. The following are examples of sampling sites:

  • food contact processing equipment, such as tables and conveyors
  • walls, floors, and drains in processing areas
  • fluid piping systems
  • vacuum and air blower systems
  • refrigeration units

Sample sites can be grouped to obtain an assessment of common areas such as:

  • food contact surfaces
  • non-food contact surfaces
  • raw ingredient handling areas
  • finished product handling areas

Food samples

Samples of a food in its finished state could be taken from a lot in storage to help assess its compliance.

Samples of food collected during preparation (to monitor characteristics such as temperature, pH or water activity) can be taken from the processing line at regular intervals (the beginning, middle and end of production).

Ingredient samples

Samples of ingredients can be taken when the ingredients are unloaded or in storage at the establishment - to help assess the effectiveness of a Supplier food safety assurance program

Water samples

To help assess the quality of the water source, water samples should be taken:

  • prior to its treatment or disinfection in the establishment
  • at a point where no further treatment is performed (usually a tap or fixture in the processing area)

5. How to sample

It is important to practice proper sampling techniques to avoid contaminating samples and exposing yourself to contaminants.

  • Wear protective items, such as gloves and protective clothing.
  • Use only clean equipment and containers to take samples.

Samples should be labelled with the information you need to link the results back to the food, ingredient or food contact surface being assessed. Sample labels should include:

  • date and time of collection
  • description of what was sampled
  • lot number
  • sampling site
  • name of the person who collected the sample

The sample units should be representative of the lot and obtained randomly:

  • each sample unit should be selected by chance and each unit in the lot should have an equal chance of being included in the sample.
  • you can use a table of random number generated using a computer software to assign a number to each unit in a lot and select units to be sampled

Collecting ingredient and food samples

When collecting samples:

  • wash and dry hands prior to sampling
  • use aseptic techniques when taking microbiological samples
  • pre-packaged food samples should be collected in an original, unopened package
  • use appropriate sampling containers that can withstand handling and shipping
  • securely seal sample containers after filling so they cannot leak or become contaminated during further handling or transportation
  • Note: Open, broken or damaged containers are not appropriate for sampling

Using aseptic techniques

When collecting samples for microbial testing avoid introducing microorganisms to the samples by following aseptic sampling procedures:

  • Use only sterile equipment and containers, and properly re-sterilize sampling tools before using them again
  • Make contact with the source material and the sample only with the sampling tool or the container
  • Use sterile gloves if a sample must be touched with the hands. An aseptic sample should not be touched with bare hands
  • Minimize exposure of the product, sampling equipment, and the interior of sampling containers to the environment. For example, avoid collecting samples in areas where dust or atmospheric conditions may contaminate the sample unless such contamination may be considered part of the sample
  • Work rapidly. open sterile sampling containers only to insert the sample, and close them immediately
  • Avoid unnecessary contact. The sample and sampling tool should not contact the interior, lip, or lid of the sterile container

Quick tip

Take care when opening and closing sample containers. It is easy to contaminate a sample during this step!

There are many things that you can do to reduce the risk of contaminating your sample. The following points are things to consider when sampling.

Opening sample containers

  • For a bottle or cup: remove the cap or lid with a free hand and keep it in that hand during sample collection; only the exterior of the cap or lid can be touched
  • For a bag: open the bag by using the tabs. Do not touch the opening of the bag
  • Do not overfill the sampling container

Closing sample containers

  • For a bottle or cup: replace the cap or lid on the container without touching the inside of the cap or the mouth of the bottle and secure the cap or lid
  • For a bag: pull twist ties tight, whirl the bag three revolutions, and fold the twist ties towards each other to seal the bag

Collecting environmental samples

When collecting environmental samples you should wear sterile gloves.

The type of surface to be sampled and lab capabilities will determine the type of swab that should be used (for example, fabric-tipped swab, swatches, sponges, gauzes and clothes).

Source water samples

  • Collect the sample from a source water outlet. Do not use a hydrant, hose, or any faucet located outside of building
  • Remove any aerators or other devices from the outlet to be sampled (These devices may be heavily contaminated and may alter the water sample result)
  • Do not sample from a tap that is obviously contaminated
  • Disinfect the end of the faucet with alcohol wipes or by flaming
  • Run the cold water for at least two to three minutes
  • Adjust the flow of the stream to ensure that no splashing occurs
  • Use a sterile cup as your sampling container
  • Securely seal sample containers after filling so they cannot leak or become contaminated during further handling or transportation

Recirculating, processing and wash water samples

  • Collect the water using a sterile cup. If sampling from a spigot, flush it out well prior to taking sample
  • Avoid contact between the cup and the equipment when taking the sample
  • Securely seal sample containers after filling so they cannot leak or become contaminated during handling or transportation

6. Storing and shipping samples

Samples for microbial testing can be compromised by the temperature they are exposed to and the time that goes by before they are tested. To preserve their integrity you should store and ship the samples at appropriate temperatures and within the time frames recommended by the laboratory.

To prevent contamination, deterioration, and other damage that could compromise the integrity of a sample during transportation:

  • before shipping, store samples in a manner to maintain their integrity
  • measure and record temperature of sample units before placing them into the shipping container
  • use sound, clean, dry shipping containers, coolers, coolant and packaging materials
  • Pack samples tightly to prevent shifting
  • transport frozen or refrigerated samples in an insulated shipping container of rigid construction and packed with suitable coolant material to maintain their frozen or refrigerated state. Avoiding direct contact with the coolant material
  • ship samples collected as quickly as possible

7. Testing the samples

Samples taken to assess the compliance of a finished product should be tested at an accredited laboratory such as a laboratory accredited to the ISO/IEC 17025 standard General requirements for the competence of testing and calibration laboratories by the:

  • Standards Council of Canada (SCC) under the Program for Accreditation of Laboratories (PALCAN), in conformity with CAN-P-4D, or
  • Canadian Association for Laboratory Accreditation (CALA)

The test should be within the scope of accreditation for the laboratory

A recognized test method should be used such as those outlined in Health Canada's Compendium of food allergen methodologies, Compendium of methods for chemical analysis of foods and Compendium of analytical methods.

In regards to marine biotoxins in shellfish, the test method used can be:

  • an official method for which the laboratory has demonstrated that they are able to achieve the published method performance parameters
  • another method that has been validated and meets Codex Alimentarius performance criteria for marine toxins

8. What to do with the results

Satisfactory results provide evidence that your control measures are effective. Unsatisfactory results however, indicate that your control measures are not effective. You are responsible for the safety of the foods you prepare and need to take action when you obtain results that indicate a food safety situation or that your control measures are not effective. It is important for you to follow up with the corrective actions that are identified in your PCP.

Tell me more! Further reading

The following references contain information that helps explain food safety controls, demonstrates how to develop them, and provides examples. The CFIA is not responsible for the content of documents that are created by other government agencies or international sources.

CFIA references

Other references

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