Quality System Procedure 142.1: Pedigreed Seed Crop Inspection Procedures - Appendices
Appendix VII: Recommended Procedures for Sampling Plants or Plant Parts for Laboratory Identification and Investigations

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While counting, describing and recording the presence of suspected deviant plants during crop inspection, there may be a need to confirm their status. These plants may be sampled and sent to the Seed Science Unit of the Ottawa Plant Laboratory - Fallowfield. Only submit samples when confirmation is required to determine if they are off-types or variants.

When possible, entire plants should be sampled. At the very least, heads or panicles including the culm and upper node of cereal crops should be sampled. When collecting and packaging plants, care must be taken to cause as little damage to the plants as possible. In order to maintain the integrity of the sample, each type of suspicious plants must be packaged separately from the sample of the norm of the variety; each package must be accurately labelled.

Soybean and bean plants should be individually wrapped in paper or plastic bags (if the plants are dry enough).

In the case of corn, one main ear from the suspected deviant plant may be harvested.

If it is suspected that there is more than one deviant type in the crop, the inspector may submit all suspected deviant plants to the Seed Science Unit, providing that they are kept separate.

The inspector must collect 5 or 6 suspected deviant plants of each type and proceed to collect and properly label a sample of five to six plants from the normal bulk of the variety in a separate envelope or paper bag. These are to be labelled as NB (normal bulk).

The inspector must include a LSTS sample submission form and a photocopy of the crop inspection report giving a brief description of the off-types. The sample code to be used is IDS. The inspector's name must be legible to assist the Seed Science Unit in forwarding results. When samples are submitted, the inspector must note on the Report of Seed Crop Inspection that samples have been submitted to the Seed Science Unit.

These samples can be shipped by Canada Post or courier, to the attention of the Seed Science Unit, Ottawa Plant Laboratory (Fallowfield), 3851 Fallowfield Rd., Building No. 210, Ottawa, Ontario K2H 8P9.

When submitting samples of varieties not registered in Canada for varietal testing, the inspector is asked to include a copy of the variety description with the sample.

Include the fax number or e-mail address to which the results should be sent.

Note: Some off-types may be confirmed on the basis of morphological characteristics while others may require some other tests in the laboratory necessitating a longer return time. The test reports are copied to the CSGA, Seed Section and the Area Program Specialist (Seeds). Casual inspectors may not receive feedback from the samples they collected due to the time frame needed for analysis.

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