Quality System Procedure 142.1: Pedigreed Seed Crop Inspection Procedures - Appendices
Appendix XII: Disease Sample Submission Procedures

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Collecting Plant Samples for Analysis

The inspector must look for areas that have just begun to show symptoms. Plants or parts of plants which have been dead too long are likely to have many organisms in them, most of which didn't cause the disease.

The inspector must take enough plant material to represent the problem and be useful. A few leaves or a stem are not enough. Generally, samples should include some roots and a little soil, even if there does not appear to be any problem in the roots.

Packing and Mailing Samples

Samples are to be sent to:

Plant Pathology Unit
Ottawa Plant Laboratory (Fallowfield)
3851 Fallowfield Rd.
PO Box 11300
Ottawa, Ontario K2H 8P9

If samples are being shipped, overnight courier service is to be used.

The inspector must pack the samples in paper or plastic Ziploc bags. However, if the samples are wet, or are already badly decomposed or infected, a plastic bag should not be used as it will encourage everything on the plant material, both pathogens and saprophytes, to grow rapidly. In this case, the samples are to be placed in a dry paper towel and in a paper bag. Leaves can be placed in a dry paper towel before placing them in a Ziploc plastic bag.

The inspector must not add any water or moisture to any samples when packing them. Samples should be placed in a cooler or box with ice packs.

If sending samples in very cold or hot times of the year, it is best not to ship them out on a Friday, as the samples may be left in a non-climate controlled condition which could result in freezing or heat spoilage of the samples.

The entire Pest Identification Report (PIR) form (CFIA/ACIA 1303) must be completed with adequate sample description.

When in doubt about what to send or how much of a sample is required for analysis, the inspector should contact the lab for advice at: Quarantine Plant Pathology Laboratory (613) 228-6690 ext 5911.

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