Listeria Environmental Sampling Program Checklist

This page is part of the Guidance Document Repository (GDR).

Looking for related documents?
Search for related documents in the Guidance Document Repository

Establishment Name:

Registration number:

Mailing Address:

Telephone number: space
Fax number:

Establishment Manager:

Quality Management Coordinator:

CFIA Reviewer:

Date of Review:

Background Product and Process Information
List all ready-to-eat products produced Category (1, 2A, 2B) Identify critical processing steps (i.e. lethality step, use of growth inhibitors, pH adjustment, etc.)

List the most likely consumers of the RTE products. Are any of them members of high-risk population groups (the elderly, pregnant women or immunocompromised individuals)?


Review comments:


CFIA Reviewer Signature: space

Date: space

Sampling procedure
1. Sampling procedure: Yes/No Comments
a description of the materials used to collect samples
a description of the procedures used to collect samples
an environmental sampling method recognized by the CFIA and prescribed in Health Canada's Compendium of Analytical Methods (MFLP-41)
Testing method
2. Testing method: Yes/No Comments
a description of the method used to test for Listeria spp. or Listeria monocytogenes
information on the lab performing the analysis (testing is conducted in and results are obtained from ISO/IEC 17025 accredited labs)
Target organism
3. Target organism: Yes/No Comments
a description of the micro organism(s) the samples are tested for (Listeria spp. or L. monocytogenes)
Sampling sites
4. Sampling sites: Yes/No Comments
a description of sites to be sampled per production line
sites consist of Food Contact Surfaces and Non-Food Contact Surfaces
a schematic of the process flow for each RTE production line (Potential sites of biological cross contamination between raw and ready-to-eat products or employee flow are identified)
a requirement for collection of at least 10 sponge/swab samples from Food Contact Surfaces before final packaging
Sampling frequency
5. Sampling frequency: Yes/No Comments
a description of when and how often environmental samples are taken (recommended frequency: category 1 = 1/week, category 2A = every other week, category 2B = 1/month)
a schedule of when to start sampling (3 hours or more after start up of operation)
a plan for when to increase the number of sample sites (i.e. under special circumstances, construction, use of previously used or modified equipment)
Sampling Site Review
6. Sampling Site Review: Yes/No Comments
a description of the process followed to regularly review the suitability of the sampling sites and the sampling frequency
provisions for instances where construction, new equipment, change in process flow, etc. could result in a loss of control for Listeria
description of what is to be done with data collected (i.e. trend analysis, QMP revisions, etc.)
Listeria spp
7. Response to Presence of Listeria spp. Yes/No Comments
a description of the corrective actions to be taken when a sample is positive for Listeria
a description of the follow-up to verify the corrective actions
a description of the response to finding Listeria spp. again
a description of the response to persistent contamination
Date modified: