Shell Egg Manual - Chapter 6 - Sampling

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Tables of Contents

6.1 Objective of Activity

Shell eggs are sampled to ensure that regulatory requirements for composition and safety are met. Operating conditions within a registered egg station are also monitored by sampling the environment for Salmonella and the cleanliness of the egg wash water.

6.2 References

Canada Agricultural Products Act
Egg Regulations
Consumer Packaging and Labelling Act and Regulations
Food and Drugs Act and Regulations
Minimum Enforcement Guidelines - (Internal access only)

6.3 Required Forms

Food Product Sampling Submission Form (CFIA/ACIA 5167)
Food Environmental Sampling Submission Form (CFIA/ACIA 5167)

* All forms listed on this page are intended for internal use. CFIA staff can access these forms using the CFIA forms catalogue - (Internal access only) and the LSTS web submission forms.

6.4 Required Equipment

Environmental sampling kits
Freezer packs
Marker for sample cups and bags
Plastic/glass sample cups - sterile
Plastic whirl pack bags - sterile
Scissors
Shipping boxes
Tamper proof tape - (CFIA/ACIA 4561)
Disinfectant - spray bottle

6.5 Chemical Residue Sampling Program - Plan E8EGG01 & E8EGG02

Frequency:

According to dates in annual sampling plans

Record:

LSTS Web Food Product Sampling Submission Form (CFIA/ACIA 5167)

Distribution:

Original to Lab
Copy filed in the CFIA Inspection Office
Copy sent to the Egg Supervisor or designate (optional)

The National Chemical Residue Monitoring Program is designed to provide a statistical determination of the presence of chemical residues in domestic and imported shell eggs. Sampling plans are distributed annually and include sampling dates for each sample number. Samples may be collected at registered egg stations, or registered processed egg stations. Many samples are sent to private labs. In the event of a positive result, the Chemical Residue Program advises the Egg Program at Headquarters of the result. This in turn is shared with the appropriate Area Egg Specialist for follow-up with inspection staff. Due to lab schedules, reports of analysis may take several months to receive.

The commodity type egg is intended for eggs in their shell. For purposes of transportation to the lab the inspector must break the shell and submit the contents to the laboratory in a sealed container, however, the product sampled must have been eggs still in their shell.

  • Domestic Sampling - For domestic samples, select one producer at a registered egg or processed egg station. Take 12 eggs, and break them into 6 sample cups (2 eggs for each 4 oz cup). Mark all 6 sample cups with the same sample number and place in the freezer. Record all pertinent information on the Food Product Sampling Submission Form (CFIA/ACIA 5167), including the producer name and barn number. Inspectors should maintain a list of producers that have been sampled to ensure different producers are sampled each time.
  • Import Sampling - For import sampling, 12 eggs are selected from an imported load of shell eggs. Take one carton or 12 eggs from the same flat, and break them into 6 sample cups (2 eggs for each 4 oz cup). Mark these sample cups with the same sample number and place in the freezer. Record all pertinent information in the Food Product Sampling Submission Form (CFIA/ACIA 5167).

Note: For completing Import Sampling, please also see Section 6.10Footnote * regarding Import Control Number vs completion of LSTS form.

The scheduled sampling date should be adhered to as much as possible because it randomizes the collection, which is especially important when only a small number of annual samples are taken. If the sampling cannot occur on the date specified, the next available lot should be sampled and the sample ID number retained for that submission. In such cases, the inspector must notify the lab of the new sampling date, usually by completion of the accompanying documentation submitted along with the sample, so that the item may be corrected in the lab data base.

It is crucial that samples are submitted to the laboratory identified in the schedule. These tests are carried out under contract and only the laboratory indicated is contracted to carry out the testing of that sample. Another laboratory would not be able to perform the testing needed.

6.5.1 Evaluation and Follow-up

Should a chemical residue be detected in a sample, a follow up should be initiated to determine the origin of the contamination. Often the Regional Feeds Officer is notified of the result and asked to aid in the investigation. This investigation may involve the feed mill that supplied the feed to the producer and result in feed samples being sent to the lab to determine if contamination with a medicated feed occurred. A report of their investigation and findings must be attached to the lab analysis and be forwarded to the Area Egg Specialist.

6.6 Environmental Sampling Program - Plan E203

Frequency:

2/year/station

Record:

LSTS Web Food Environmental Sampling Submission Form (CFIA/ACIA 5167)

Distribution:

Original to Lab
Copy filed in the CFIA Inspection Office
Copy sent to the Egg Supervisor or designate (optional)

Shell eggs, because of their nature and composition, are considered to be potential carriers of Salmonella, particularly if the eggs are subjected to improper handling.

Numerous studies have revealed that freshly laid eggs are relatively bacteria free. The subsequent increase in bacteria load after laying results from variations in the environment surrounding the egg during the gathering, processing and handling stages.

Our interest in determining the frequency and types of Salmonella found on or in shell eggs stems from two different but related concerns. The first relates to the identification and control of Salmonella, particularly Salmonella enteritidis on producer premises in Canada. The second concerns public health and the control of Salmonella to protect consumer packs from pathogen contamination.

The shell of an undamaged egg provides an extremely efficient barrier to microbial invasion. However, micro-organisms, including Salmonella, can harbour in or on the surface of the shell, in the egg contents of improperly handled eggs or possibly by ovarian transmission.

The concept of Environmental Sampling as applied to an egg station, as in any food handling and processing environment, is aimed at the discovery of the most probable sites that can harbour Salmonella. Detection of these sites in the station environment will provide us with assistance as to where to concentrate our efforts with regards to plant sanitation programs.

6.6.1 Objectives of the Environmental Plan

  • To verify the sanitation program of the egg station and their ability to control the presence of Salmonella.
  • By follow-up sampling when positives are detected, to demonstrate that adjusted sanitation programs are effective.
  • To identify the extent of Salmonella contamination in egg grading facilities.
  • To identify the probable cross-contamination sites (critical control points) for control purposes.
  • To identify non-quota Salmonella enteritidis positive flocks.

6.6.2 Sampling Procedure

Sampling should be performed before the start-up of the day's operation or after a post operation cleaning.

The environmental sampling should take about two hours to complete at an egg station, depending on its size. About half of this time should be spent preparing a work plan. This involves scheduling the sampling, checking the sampling kit, and selecting sample sites at the station.

The scheduling of the kits will be determined in consultation with the laboratory. They should be arranged several months in advance. When the kit is received, it should be checked to ensure that it is complete. Also, before leaving for the station, check to ensure that you have all other necessary equipment. (Sampling kits should be used by their best before date, so inspectors should use their oldest kits first)

The environmental sampling kit assembled by the lab will include the following items:

  • pre-moistened sponges
  • whirl-pack bags
  • sterile gloves
  • insulated cooler and freezer packs

The inspector must have the following equipment before starting the sampling procedure:

  • clean coveralls or lab coat
  • clean footwear
  • permanent marking pen for sample identification
  • Food Environmental Sampling Submission Form (CFIA/ACIA 5167) from LSTS.

Using the guidelines, decide which sites will be sampled and exactly where at the site the sample will be taken.

For each sample number, two composites of five swabs each will be sent to the lab. Inspectors should always select one composite from the Graded Product Contact Surfaces (as indicated in the Table on page 4) and one composite from one of the other three boxes on an alternating basis (graded product non-contact surfaces, ungraded product contact surfaces, and ungraded non-contact surfaces). Please note: the sites listed below are examples only. Sites that are not included may still be sampled.

For non-quota holders that are not tested by the Egg Farmers of Canada's flock sampling program, the ungraded area samples should include sites that may detect the presence of Salmonella in the barn. (e.g. ante-room, ungraded egg packaging)

Suggested Environmental Sampling Sites

Graded Product Contact Surfaces

  • Washer brushes
  • Washer rollers
  • Egg dryer
  • Crack detector
  • Scales
  • White belt on scales
  • Towel or belt
  • Packing head (transfer point)
  • Packing table
  • Clam shells
  • Carton conveyor
  • Suction cups at packing
  • Plastic trays

Ungraded Product Contact Surfaces

  • Farm Carts
  • Pre loader suction cups
  • Conveyor rollers/belts
  • Plastic trays
  • Wood/plastic pallets or dividers

Graded Product Non-Contact Surfaces

  • Graded egg cooler floor/door/walls
  • Graded cooler condensing unit
  • Carts leaving station
  • Dry storage door

Ungraded Non-Contact Surfaces

  • Ungraded cooler floor/door/walls
  • Buffer/ante room floor/door/walls
  • Pre loader frame
  • Hand wash sink

6.6.4 Sampling Technique

Inspectors must wear clean coveralls or lab coats and wash and sanitize their hands before beginning. Rings and watches should be removed.

Under this program 10 samples are to be collected from the egg station. Five swabs should be used for the graded product contact surfaces area and five from one of the other three boxes. (See section Footnote *) Use a permanent marker to write the sample number on each sample bag as well as the description of the site that is swabbed, followed by the word ungraded or graded as applicable. The sample number that is written on each bag should end with 01 (graded) or 02 (ungraded) to clearly identify which composite the swabs belong to.

Care should be taken to only touch the sponge with a sterile gloved hand. Using the dampened sponge, wipe the area to be sampled. The surface area swabbed is generally about 900 square centimetres. (30 cm x 30 cm) Place the sponge in a labelled whirl pack bag. Seal the bag tightly and ensure that it cannot leak and the contents cannot become contaminated during shipping.

The samples must be cooled as quickly as possible to temperatures between 0°C and 10°C and maintained at this temperature until received by the laboratory. Ship all samples to the laboratory in thick walled coolers, using freezer packs on top to completely fill the cooler. Frozen environmental samples will not be accepted for lab analysis. Samples must be received at the lab within 48 hours of sampling. The completed laboratory report should also be sent in the cooler.

Note: To avoid unnecessary re-sampling, additional cooler packs (hard plastic packs are more effective than foam packs) need to be added during warm weather to ensure that the samples do not arrive at the lab temperature compromised.

A Food Environmental Sampling Submission Form (CFIA/ACIA 5167) is to be completed in the LSTS

  • In the Sample No. Field of the form; list the sample numbers including 01 or 02, referring to the composite to which it belongs. The first five swabs listed end with 01 while the second five end with 02.
  • Write the description of the site sampled, including whether the site was ungraded or graded,

An Egg Station Inspection/Rating Report (CFIA/ACIA 3761) should be completed while at the station. This will provide necessary background information in the case of a positive Salmonella.

6.6.5 Evaluation and Follow-up - Plan E203D

  • If Salmonella is identified in the egg station, steps must be taken to eliminate or control the hazard. The egg station operator must be immediately notified that one or both of the composites have tested positive for Salmonella. The station operator is asked to thoroughly clean the egg station prior to a follow up environmental sampling, and to take corrective actions to prevent a reoccurrence.
  • Follow-up sampling should not be carried out using a number from the original list of sample numbers. A special follow-up number is to be obtained through the Regional Program Officer or Area Egg Specialist.
  • The inspector returns to the egg station as soon as possible after the corrective action have been completed and performs the follow-up sampling that will consist of about 5-10 samples, depending on whether one or both composites tested positive. These follow up samples will be analyzed individually by the lab so that the contamination site can be identified.
  • In the event one or more of the individual follow-up samples is found to be positive, the lab result will be assessed as unsatisfactory. The egg station operator must be immediately notified and an Action Plan Poultry/Egg Programs (CFIA/ACIA 2280) outlining the steps that will be taken to address this second positive, must be submitted to the inspector. The failure of the follow-up sample indicates that the initial corrective actions taken by the station were not effective, and therefore, the operator should, as a minimum, consider the following review as part of the action plan: sanitation program, employee traffic patterns and pest control program. The station must perform a thorough cleaning prior to follow up sampling.
  • The inspector returns to the egg station as soon as possible after the corrective actions have been completed and perform a follow-up sampling. If only one site was found positive for Salmonella, that site is re-swabbed along with four others to a maximum of five swabs. If more than one site was found positive, those sites are re-swabbed along with additional sites to a maximum of ten swabs.
  • The registration status of the egg station will be reviewed if the egg station's action plan is not followed as outlined and positive results continue.
  • Environmental follow ups are subject to cost recovery charges. See Chapter 12 -Shell Egg - Cost Recovery for additional information.

6.7 Wash Water Sampling Program - Plan E202

Frequency:

Stations with recirculating washer's 4/year/station
Stations with basket washer's 2/year/station

Record:

LSTS Web Food Environmental Sampling Submission Form (CFIA/ACIA 5167)

Distribution:

Original to Lab
Copy filed in the CFIA Inspection Office
Copy sent to the Egg Supervisor or designate (optional)

The purpose of the wash water sampling program is to determine if an egg station has an acceptable method for controlling the quality of the water used for washing eggs. An acceptable method is one where the washer is clean, and the eggs are being washed in water that will effectively clean off any adhering dirt without causing contamination in the process.

This is generally achieved by keeping the aerobic colony counts (ACC) in the wash water below 100,000/g, by maintaining a clean washer, and by maintaining a wash water temperature of 40°C (104°F) or more, and a pH level of at least 10.

6.7.1 Recirculating Washers

Frequency

In a new station, collect 3 samples per tank during the first week of operation. When a new recirculating egg washer is installed, where possible, collect 3 samples per tank during the first week of operation of the new equipment.

For the regular monitoring of stations collect 3 samples per tank, once per quarter in all stations.

Sampling Method

  • Using an 8 oz sterile sample cup, collect the egg wash water from the re-circulating tank by ladling the wash water into the sample cup and filling to the 6 oz mark. If the operator has a spigot on the tank for drawing samples flush it out well prior to taking the sample.
  • Take the 3 samples at well-spaced intervals during the operation. Water temperature and pH measurements should be recorded on the lab submission form. Inspectors should note if dirty eggs are being washed or eggs are breaking during washing, when samples are being taken. This should be recorded in the comments section of the Food Environmental Sampling Submission Form CFIA/ACIA 5167
  • The samples must be cooled as quickly as possible to temperatures between 0°C and 10°C and maintained at this temperature until received by the laboratory. Thick walled Styrofoam coolers filled with freezer packs are to be used for shipping samples to the lab. Samples must be received at the lab within 48 hours of sampling.

6.7.2 Basket Washers

Frequency

For preliminary monitoring of egg wash water used in basket type washers, collect 3 samples the first day of operation.

For regular monitoring of stations using basket type washers, collect 1 sample per visit at a frequency of twice per year.

Sampling Method

  • Samples should be taken by carefully pouring water from the basket washer into an 8 oz sterile sample cup. The top portion of the opened cup must not touch the basket washer during the sampling procedure. If you inadvertently touch the washer, the sample is to be discarded. Water temperature and pH measurements should be recorded on the lab submission form.
  • The samples must be cooled as quickly as possible to temperatures between 0°C and 10°C and maintained at this temperature until received by the laboratory. Thick walled Styrofoam coolers filled with freezer packs are to be used for shipping samples to the lab. Samples must be received at the lab within 48 hours of sampling.

6.7.3 Aquamagic Washers

Wash water sampling is not required on Aquamagic type machines because the water is not re-circulated.

6.7.4 Evaluation and Follow-up

Wash water results in excess of 100,000/g

  • A lab result that indicates that the wash water aerobic colony count (ACC) from the pre-washer or any wash tank was greater than 100,000/g will be assessed as unsatisfactory.
  • The inspector notifies the egg station operator of the unsatisfactory results and asks them to take corrective actions to prevent a reoccurrence.
  • The inspector is to obtain a follow-up sample number (under the sampling plan E202D) from the Regional Program Officer or Area Egg Specialist.
  • The inspector returns to the egg station as soon as possible after the corrective actions have been completed. The inspector completes an Egg Station Inspection/Rating Report (CFIA/ACIA 3761) and removes 20 points for the wash water failure (ACC > 100, 000/g).
  • During the return visit to the station, the inspector is to collect 3 follow-up samples of the wash water and submit them to the lab under sampling plan E202D. Each of these samples will be analyzed individually and all 3 results will be reported.
  • In the event that one or more of the individual follow-up samples fails, the lab result will be assessed as unsatisfactory.
    • If the follow-up is unsatisfactory for ACC > 100 000/g :
      • The egg station operator must be notified and an Action Plan Poultry/Egg Programs CFIA/ACIA 2280 must be submitted to the inspector. The failure of the follow-up sample indicates that the initial corrective actions taken by the station were not effective. Therefore, when developing the action plan, the operator should, as a minimum, consider taking the following actions: a cleaning and acid bath of the washer, evaluation of the pH and water temperature and evaluation of how often the wash water is changed.
      • The inspector returns to the egg station as soon as possible after the corrective actions have been completed. The inspector completes an Egg Station Inspection/Rating Report (CFIA/ACIA 3761) and removes 20 points for the follow-up wash water failure (ACC > 100,000/g). During the return visit to the station, the inspector is to collect 3 follow-up samples of the wash water and submit them to the lab under sampling plan E202D. Each of these samples will be analyzed individually and all 3 results will be reported.
    • If the follow-up is unsatisfactory for ACC > 1 000 000/g
      • The egg station operator must be notified. The failure of the follow-up sample indicates that the corrective actions taken by the station were not effective. The egg station operator must submit an Action Plan Poultry/Egg Programs (CFIA/ACIA 2280) to the inspector outlining what actions will be taken to correct their wash water failure. The inspector returns to the egg station, completes an Egg Station Inspection/Rating Report (CFIA/ACIA 3761), removes 20 points for follow-up failure (ACC > 1,000,000/g), assigns a Serious sanitation rating, and assigns 5 demerits.
  • The registration status of the egg station will be reviewed if the egg station's action plan is not followed as outlined and/ or wash water failures continue.
  • Wash water follow ups are subject to cost recovery charges. See Chapter 12 - Shell Egg -Cost recovery.

Wash water results in excess of 1,000,000/g

  • A lab result that indicates that the wash water aerobic colony count (ACC) from the pre-washer or any wash tank was greater than 1,000,000/g will be assessed as unsatisfactory.
  • The inspector notifies the egg station operator of the unsatisfactory results. The egg station operator must submit an Action Plan Poultry/Egg Programs (CFIA/ACIA 2280) to the inspector outlining what actions will be taken to correct their wash water failure.
  • The action plan should include, as a minimum, the following : a cleaning and acid bath of the washer, an evaluation of the pH and water temperature and an evaluation of how often the wash water is changed.
  • The inspector should obtain a follow up sample number from the Regional Program Officer or Area Egg Specialist.
  • The inspector returns to the egg station as soon as possible after the corrective actions have been completed. The inspector completes an Egg Station Inspection/Rating Report (CFIA/ACIA 3761), removes 20 points for the wash water failure (ACC >1,000,000/g), assigns a Serious sanitation rating, and assigns 5 demerits.
  • During the return visit to the station, the inspector is to collect 3 follow-up samples of the wash water and submit them to the lab under sampling plan E202D. Each of these samples will be analyzed individually and all 3 results will be reported.
  • If any one of the individual follow-up samples fails the lab result will be assessed as unsatisfactory.
  • If the follow-up is unsatisfactory for ACC > 100 000/g :
    • The egg station operator must be notified and an Action Plan Poultry/Egg Programs (CFIA/ACIA 2280) must be submitted to the inspector. The failure of the follow-up sample indicates that the initial corrective actions taken by the station were not effective. Therefore, when developing the action plan, the operator should, as a minimum, consider taking the following actions: a cleaning and acid bath of the washer, evaluation of the pH and water temperature and evaluation of how often the wash water is changed.
    • The inspector returns to the egg station as soon as possible after the corrective actions have been completed. The inspector completes an Egg Station Inspection/Rating Report (CFIA/ACIA 3761) and removes 20 points for the follow-up wash water failure (ACC >100,000/g). During the return visit to the station, the inspector is to collect 3 follow-up samples of the wash water and submit them to the lab under sampling plan E202D. Each of these samples will be analyzed individually and all 3 results will be reported
  • If the follow-up is unsatisfactory for ACC > 1 000 000 /g :
    • The egg station operator must be notified. The failure of the follow-up sample indicates that the action plan implemented by the station was not effective. The egg station operator must submit an Action Plan Poultry/Egg Programs (CFIA/ACIA 2280) to the inspector outlining what actions will be taken to correct their wash water failure. The inspector returns to the egg station, completes an Egg Station Inspection/Rating Report (CFIA/ACIA 3761), removes 20 points for follow-up failure (ACC >1,000,000/g), assigns a Serious sanitation rating, and assigns 5 demerits. The inspector should also inform the operator about the likelihood of enforcement action, if wash water failures continue to reoccur.
    • The registration status of the egg station will be reviewed if the egg station's action plans are not followed as outlined and/ or wash water failures continue.
    • Wash water follow ups are subject to cost recovery charges. See Chapter 12- Shell Egg -Cost recovery for additional information.

6.8 Composition Sampling Program (Cholesterol, Fatty Acid and Vitamin E Testing) - Plan E104

Frequency:

According to dates in the annual sampling plan

Record:

LSTS Web Food Product Sampling Submission Form (CFIA/ACIA 5167)

Distribution:

Original to Lab
Copy filed in the CFIA Inspection Office
Copy sent to the Egg Supervisor or designate (optional)

Sampling Instructions for Shell Eggs - Monitoring- E104

Shell eggs that have omega-3, DHA, cholesterol or Vitamin E claims should be sampled under this sampling plan. (DHA is Docosahexaenoic acid which is an omega-3 essential fatty acid.)

1. In order to ensure random sampling, the following procedure should be followed:

  • From the lot of eggs to be sampled, select twelve (12) units of eggs at random using the random numbers table (a unit is typically a box).
  • Select one (1) carton of eggs from each of the twelve units. Cartons should be selected from different positions in each unit.
  • Select one (1) egg randomly from each of the twelve cartons. The twelve eggs will constitute one sample.

2. Break the twelve (12) eggs into a suitable container (plastic sample cups or screw cap Mason jars are both acceptable). Do not lose any part of the yolk or the white. Ensure that the sample is well homogenized. Seal the sample with tamper proof tape. Freeze immediately and ship in the frozen state.

3. Ensure that the LSTS sample submission form has been accurately filled out. For monitoring samples, the sampling plan number is E104. Include the producer premises code information, the best before date and the correct brand name and any quantitative claims made outside the Nutrition Facts Table (e.g. 0.085g DHA per egg).

Important: Send a copy of the label to the laboratory with the sample.

4. Ship the samples with freezer packs using overnight delivery to:
Canadian Food Inspection Agency
Longueuil Laboratory - Chemistry (Food)
1001 rue Saint-Laurent Ouest
Longueuil QC J4K 1C7

5. At the laboratory, the sample will be analyzed for total fat (triglycerides), cis-Monounsaturated fatty acids, cis-Polyunsaturated fatty acids, saturated fatty acids, Omega-3 fatty acids, Omega-6 fatty acids and trans fatty acids, based on AOAC 996.06 and for cholesterol based on AOAC 994.10., and the level of DHA. For Vitamin E testing the method used is LCAQ-094 based on the Journal of Food Composition and Analysis.

Sampling instructions for Shell Eggs - Directed (follow-up) E104D

When laboratory results are found to be unsatisfactory for cholesterol, Omega-3 fatty acids, Vitamin E or DHA, a follow-up sample should be taken. The inspector notifies the station operator of the unsatisfactory result and asks them to take corrective action.

Where laboratory results are found to be unsatisfactory for any other of the tests (i.e. total fat, cis-monounsaturated fatty acids etc.), a follow up sample does not need to be taken, however, the plant should be advised of our results and their own composition testing results should be reviewed. The plant is responsible for labelling their products accurately.

Where a follow up is required, the following steps should be taken:

1. A follow-up sample number should be obtained from the Regional Program Officer or Area Egg Specialist.

2. In order to ensure random sampling, the following procedure should be followed:

  • From the appropriate lot of eggs, select twelve (12) units of eggs at random using the random numbers table (a unit is typically a box).
  • Select one (1) carton of eggs from each of the twelve units. Cartons should be selected from different positions in each unit.
  • Now select three (3) eggs randomly from each of the twelve cartons (for a total of 36 eggs), and place them into 3 separate sub-sample portions. The first eggs selected from each carton become sub-sample A. The second egg selected from each carton becomes sub-sample B and the third egg selected from each carton becomes sub-sample C.
  • These 3 sub-samples should be labelled with the follow-up sample number and carry the suffix A, B and C respectively.

3. Break each of the three one-dozen egg sample portions into separate containers of the appropriate size (plastic sample cups or screw cap Mason jars are both acceptable). Do not lose any part of the yolk or the white. Ensure that the samples are well homogenized. Seal the samples with tamper proof tape. Freeze samples and ship in the frozen state. The inspector is responsible for the sample continuity from the time of the selection of the samples to the time that the samples are shipped.

4. Ensure that the LSTS sample submission form has been accurately filled out. For follow-up samples, the sampling plan number is E104D. Include the producer premises code information, the best before date and the correct brand name and any quantitative claims made outside the Nutrition Facts Table (e.g. 0.085g DHA per egg). The original sample number that triggered the follow-up sample should be included in the comments section of the lab submission form. Send a copy of the label to the laboratory with the sample.

5. Notify the laboratory that a follow-up sample will be arriving under the E104D sampling plan. Ship the samples with freezer packs using overnight delivery to:

Canadian Food Inspection Agency
Longueuil Laboratory - Chemistry (Food)
1001 rue Saint-Laurent Ouest
Longueuil QC J4K 1C7

6. The laboratory Report of Analysis for E104D follow-up samples will show individual results for each of the three sub-samples, as well as the mean. The assessment will be made based on the Nutrition Labelling Compliance Test.

When results from the follow-up sample are unsatisfactory for cholesterol, Omega-3, Vitamin E or DHA, appropriate enforcement action should be taken. Refer to the Minimum Enforcement Guidelines (MEGS) - (Internal access only). on Merlin.

Omega-3, DHA, Vitamin E or cholesterol follow ups are subject to cost recovery charges. See Chapter 12- Shell Egg -Cost recovery for additional information.

6.9 Imported Shell Egg Sampling Program - Plan E207

Frequency:

Every import load of table eggs is to be sampled according to sampling plan

Record:

LSTS Web Food Product Sampling Submission Form (CFIA/ACIA 5167)

Distribution:

Original to Lab
Copy filed in the CFIA Inspection Office
Copy sent to the Egg Supervisor or designate (optional)

From every import of table eggs, 24 eggs shall be randomly selected from throughout the load, packed in cartons, and sent to the laboratory. Eggs are to be sent to the laboratory intact and are not to be frozen. These eggs are analyzed for Salmonella enteritidis. A load containing eggs from different grading stations should be sampled separately using separate sampling numbers.

6.9.1 Evaluation and Follow-up

Should a sample be found positive for Salmonella enteritidis, the Area Egg Specialist is to be notified immediately to evaluate if further actions are warranted. The inspector would need to find out where the eggs had been sent to in Canada. The United States Department of Agriculture (USDA) would immediately be notified of the positive result and source of the USA eggs by the Chief, Egg Programs, Ottawa. Eggs from that USA egg grading station would not be accepted into Canada until the USDA had taken corrective actions at the farm and subsequent environmental sampling was found to be negative for Salmonella enteritidis.

Frequency:

Every import load of table eggs to be sampled

Sampling Plans:

Chemical Residue (E8EGG02) & Shell Egg Sampling Program (E207)

Records:

ICTS & LSTS

When product is sampled under the Imported Chemical Residue (E8EGG02) or Imported Shell Egg Sampling Plans (E207), all the sample information (including Import Control Number) must be entered in LSTS. Only after this action is completed and the LSTS form submitted, should the status of the Import shipment in ICTS be changed from Aw inspection (awaiting inspection) to Processing complete.

Appendix I - Sampling Plans for Shell Eggs

Table description

This table lists the name and descriptions of the sampling plans for shell eggs.

Sample Plan ID Suggested Description in LSTS
E104 Shell Egg Cholesterol and Vitamin E Content, Fatty Acid Composition
E104D Shell Egg Cholesterol and Vitamin E Content, Fatty Acid Composition - Directed
E202 Shell Egg Wash Water
E202D Shell Egg Wash Water - Directed
E203 Shell Egg Grading Station Environmental Sampling
E203D Shell Egg Grading Station Environmental Sampling - Directed
E207 Shell Egg Micro - Imports
E8EGG01 Shell Egg Chemical Residues - Domestic
E8EGG01D Shell Egg Chemical Residues - Domestic Directed
E8EGG02 Shell Egg Chemical Residues - Imports
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