Denomination: 'PA4CN185'
Botanical Name: Brassica napus
Applicant/Holder: Bayer CropScience Inc.
Site 600, R.R. #6
P.O. Box 117
Saskatoon, Saskatchewan
S7K 3J9
Breeder: Stewart Brandt, Bayer CropScience Inc., Saskatoon, Saskatchewan
Application Date: 2015-06-23
Application Number: 15-8664
Grant of Rights Date: 2017-08-25
Certificate Number: 5534
Exemption from compulsory licensing: Yes
Expiry date for exemption from compulsory licensing: 2019-08-25
Grant of Rights Termination Date: 2037-08-25

Note: The applicant has requested an exemption from compulsory licensing to allow time to multiply and distribute propagating material of the variety. If the exemption is granted, it may be allowed for two years from the date rights are granted for the variety.

Variety Description

Varieties used for comparison: 'PA1CN131', 'PPS01-140 A-Line' and '5440'

Summary: The cotyledon of 'PA4CN185' is narrower and shorter than those of 'PA1CN131' and '5440'. The leaf of 'PA4CN185' is shorter than the reference varieties and it is narrower than those of 'PA1CN131' and '5440'. 'PA4CN185' has a shorter petiole than those of 'PPS01-140 A-Line' and '5440'. The petal of 'PA4CN185' is shorter than that of '5440' and it is larger than those of 'PA1CN131' and 'PPS01-140 A-Line'. The silique of 'PA4CN185' is longer than those of the reference varieties. 'PA4CN185' has a longer beak than those of 'PA1CN131' and 'PPS01-140 A-Line'. The pedicel of 'PA4CN185' is shorter than that of '5440'. At maturity, the plant of 'PA4CN185' is taller than the reference varieties. The seed coat of 'PA4CN185' is black whereas it is brown for 'PA1CN131'.


PLANT: male sterile inbred line, spring type, medium to tall at maturity

COTYLEDON: narrow, short to medium length

LEAF: medium green, few to medium number of lobes, rounded margin, medium to dense density of shallow to medium depth margin dentations, very short, very narrow, very short to short petiole

FLOWER PETAL: yellow, medium length and width

SILIQUE: semi-erect to horizontal attitude, medium to long, short to medium beak, very short to short pedicel
SEED COAT: black

AGRONOMIC CHARACTERISTICS: medium resistance to lodging
QUALITY CHARACTERISTICS: erucic acid is 0.02% of total fatty acids, oil content is 44.9% of whole dried seed, protein is 44.5% of dried oil free meal, low glucosinolates (14.8 µmol/g)
DISEASE REACTION: resistant to Blackleg (Leptosphaeria maculans asexual stage: Phoma lingam)

Origin & Breeding History: 'PA4CN185' is a male sterile line which contains the Ms8 gene construct in the heterozygous state. It was derived by backcrossing a double haploid line to a male sterile line used as the source of the bar and barnase genes. The double haploid line was extracted from the F1 generation derived from a cross made in 2009 at Bayer CropScience in Saskatoon, Saskatchewan, Canada and was produced in 2010. 'PA4CN185' was selected in 2013 and 2014 on the basis of male sterility stability, expression of tolerance to glufosinate-ammonium herbicide and good combining ability with numerous restorer lines. Other selection parameters included height, vigour, maturity, blackleg resistance, oil content, fatty acid profile and glucosinolate content.

Tests & Trials: The comparative trials conducted during the summers of 2014 and 2015 in Saskatoon, Saskatchewan were set up in a RCB Design with 3 replications per variety. Each year, the plots consisted of 3 rows that were 6 metres in length with a spacing of 25 cm between rows and 50 cm between plots. The measured characteristics were based on 30 measurements of the cotyledon, leaf, flower and plant height and 60 measurements of the silique. The means were based on a two year average. Mean differences were significant at the 2% probability level based on LSD values.

Comparison tables for 'PA4CN185' with reference varieties 'PA1CN131', 'PPS01-140 A-Line' and '5440'

Cotyledon length (mm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=1.5) 9.7 11.3 9.6 13.9
std. deviation 0.7 0.9 1.7 1.1

Cotyledon width (mm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=0.9) 19.2 22.0 18.9 24.8
std. deviation 1.6 1.2 2.9 1.6

Leaf length (cm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=1.5) 14.0 16.2 17.6 20.5
std. deviation 1.8 1.3 1.8 2.8

Leaf width (cm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=0.6) 7.0 8.8 8.4 9.1
std. deviation 1.3 1.0 1.1 1.2

Petiole length (cm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=0.8) 6.5 6.3 8.3 9.5
std. deviation 1.8 1.0 1.7 2.6

Flower petal length (mm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=1.0) 14.1 9.7 11.0 15.5
std. deviation 2.0 1.5 1.3 2.2

Flower petal width (mm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=0.7) 6.9 4.9 5.9 7.5
std. deviation 1.3 0.9 0.9 1.5

Silique length (mm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=3.4) 65.6 46.2 56.8 61.0
std. deviation 6.0 9.0 5.6 4.7

Beak length (mm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=1.2) 11.2 8.7 8.0 10.8
std. deviation 2.0 2.3 2.2 1.8

Pedicel length (mm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=1.5) 13.9 12.6 14.0 22.0
std. deviation 2.9 3.3 3.1 5.6

Plant height at maturity (cm)

  'PA4CN185' 'PA1CN131' 'PPS01-140 A-Line' '5440'
mean (LSD=5.2) 135 120 125 127
std. deviation 12 11 10 11

Click on image for larger view
Canola: 'PA4CN185' (far left) with reference varieties 'PPS01-140 A-Line' (centre left), 'PA1CN131' (centre right) and '5440' (far right)

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