Denomination: 'PB2CN256'
Botanical Name: Brassica napus
Applicant/Holder: Bayer CropScience Inc.
Site 600, R.R. #6
P.O. Box 117
Saskatoon, Saskatchewan
S7K 3J9
Breeder: Stewart Brandt, Bayer CropScience Inc., Saskatoon, Saskatchewan
Application Date: 2014-06-19
Application Number: 14-8363
Protective direction granted: 2014-06-19
Grant of Rights Date: 2016-05-25
Certificate Number: 5352
Exemption from compulsory licensing: Yes
Expiry date for exemption from compulsory licensing: 2018-05-25
Grant of Rights Termination Date: 2036-05-25

Note: The applicant has requested an exemption from compulsory licensing to allow time to multiply and distribute propagating material of the variety. If the exemption is granted, it may be allowed for two years from the date rights are granted for the variety.

Variety Description

Varieties used for comparison: 'PB0CN216', 'PPS01-140 B-Line' and '5440'

Summary: The cotyledon of 'PB2CN256' is larger than that of 'PPS01-140 B-Line'. 'PB2CN256' has few leaf lobes whereas 'PB0CN216' has many. The leaf of 'PB1CN256' is shorter than that of 'PB0CN216'. 'PB2CN256' has a shorter petiole than 'PB0CN216' and 'PPS01-140 B-Line'. 'PB2CN256' flowers earlier than the reference varieties. The petal of 'PB2CN256' is smaller than that of 'PB0CN216' whereas it is longer than that of 'PPS01-140 B-Line'. 'PB2CN256' has a shorter silique than 'PB0CN216'. 'PB2CN256' has a longer silique beak and pedicel than 'PPS01-140 B-Line'. 'PB2CN256' matures earlier than 'PB0CN216'. 'PB2CN256' is susceptible to glufosinate ammonium herbicides whereas '5440' is resistant.


PLANT: male fertile inbred line, spring type, medium height at maturity

COTYLEDON: wide to very wide, medium to long

LEAF: medium green, few lobes, rounded to sharp margin, medium density of medium depth margin dentations, short, narrow to medium width, very short to short petiole

FLOWER PETAL: yellow, medium length and width

SILIQUE: semi-erect attitude, medium length, medium length beak, short to medium length pedicel
SEED COAT: black

AGRONOMIC CHARACTERISTICS: fair resistance to lodging
QUALITY CHARACTERISTICS: 0.03% erucic acid, oil content is 48.5% of whole dried seed, protein is 44.7% of dried oil free meal, low glucosinolates (13.9 µmol/g)
CHEMICAL REACTION: susceptible to glufosinate ammonium herbicides
DISEASE REACTION: moderately resistant to Blackleg (Leptosphaeria maculans asexual stage: Phoma lingam)

Origin & Breeding History: 'PB2CN256' is the male fertile maintainer line of 'PA2CN156'. It was derived by backcrossing a pure selected line to a male sterile line used as the source of the bar and barnase genes. The pure selected line was backcrossed in Saskatoon, Canada in 2003. The final backcross was made in 2011. 'PB2CN256' was selected in 2012 for plant height, vigour, maturity, blackleg resistance, oil content, linolenic acid content, fatty acid profile and glucosinolate content.

Tests & Trials: The comparative trials conducted during the summers of 2013 and 2014 in Saskatoon, Saskatchewan were set up in a RCB Design with 3 replications per variety. Each year, the plots consisted of 3 rows that were 6 metres in length with a spacing of 25 cm between rows and 50 cm between plots. The measured characteristics were based on 30 measurements of the cotyledon, leaf, flower and plant height and 60 measurements of the silique. The means were based on a two year average. Mean differences were significant at the 2% probability level based on LSD values.

Comparison tables for 'PB2CN256' with reference varieties 'PB0CN216', 'PPS01-140 B-Line' and '5440'

Cotyledon length (mm)

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean (LSD=2.1) 14.3 15.2 12.6 13.9
std. deviation 1.0 1.2 1.0 1.3

Cotyledon width (mm)

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean (LSD=2.7) 28.3 27.2 23.1 25.7
std. deviation 1.9 2.0 1.8 1.7

Leaf length (mm)

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean (LSD=11) 192 210 195 216
std. deviation 14 13 16 12

Petiole length (mm)

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean (LSD=6) 68 79 77 85
std. deviation 10 11 11 6

Days to flower

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean 38 43 40 40

Flower petal length (mm)

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean (LSD=0.7) 14.4 15.4 12.6 13.8
std. deviation 0.5 0.6 0.8 0.6

Flower petal width (mm)

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean (LSD=0.4) 6.2 7.5 6.1 6.0
std. deviation 0.5 0.5 0.5 0.6

Silique length (mm)

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean (LSD=2.5) 62.4 74.8 59.7 64.4
std. deviation 5.7 3.3 3.5 4.1

Beak length (mm)

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean (LSD=1.2) 12.5 12.8 7.0 11.9
std. deviation 1.4 1.6 1.3 0.9

Pedicel length (mm)

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean (LSD=1.6) 23.0 20.5 18.6 24.0
std. deviation 3.0 3.1 2.7 5.3

Days to maturity

  'PB2CN256' 'PB0CN216' 'PPS01-140 B-Line' '5440'
mean 96 98 96 96

Click on image for larger view
Canola: 'PB2CN256' (far left) with reference varieties 'PPS01-140 B-Line' (centre left), 'PB0CN216' (centre right) and '5440' (far right)

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