Denomination: 'PA3CN164'
Botanical Name: Brassica napus
Applicant/Holder: Bayer CropScience Inc.
Site 600, R.R. #6
P.O. Box 117
Saskatoon, Saskatchewan
S7K 3J9
Breeder: Stewart Brandt, Bayer CropScience Inc., Saskatoon, Saskatchewan
Application Date: 2014-06-19
Application Number: 14-8362
Protective direction granted: 2014-06-19
Grant of Rights Date: 2016-05-25
Certificate Number: 5351
Exemption from compulsory licensing: Yes
Expiry date for exemption from compulsory licensing: 2018-05-25
Grant of Rights Termination Date: 2036-05-25

Note: The applicant has requested an exemption from compulsory licensing to allow time to multiply and distribute propagating material of the variety. If the exemption is granted, it may be allowed for two years from the date rights are granted for the variety.

Variety Description

Varieties used for comparison: 'PA0CN116', 'PPS01-140 A-Line' and '5440'

Summary: The cotyledon of 'PA3CN164' is smaller than that of '5440'. The leaf of 'PA3CN164' has a rounded margin with a medium number of lobes whereas the leaf of 'PA0CN116' has a sharp margin with many lobes. 'PA3CN164' has a narrower leaf and a shorter petiole than '5440'. 'PA3CN164' flowers later than '5440'. The petal of 'PA3CN164' is shorter and narrower than that of the reference varieties. The silique of 'PA3CN161' is shorter than that of the reference varieties. The silique beak of 'PA3CN161' is longer than that of 'PPS01-140 A-Line' whereas it is shorter than that of 'PA0CN116' and '5440'. The pedicel of 'PA3CN164' is shorter than that of '5440'. At maturity, the plant of 'PA3CN164' is taller than that of '5440'. 'PA3CN164' is later maturing than 'PPS01-140 A-Line' and '5440'.


PLANT: male sterile inbred line, spring type, tall to very tall at maturity

COTYLEDON: narrow to medium width, medium length

LEAF: medium green, medium number of lobes, rounded margin, low to medium density of shallow margin dentations, very short to short, narrow, very short to short petiole

FLOWER PETAL: yellow, short to medium length, narrow to medium width

SILIQUE: semi-erect attitude, short, short to medium beak, very short to short pedicel
SEED COAT: black

AGRONOMIC CHARACTERISTICS: medium to good resistance to lodging
QUALITY CHARACTERISTICS: 0.01% erucic acid, oil content is 46.4% of whole dried seed, protein is 37.9% of dried oil free meal, very low glucosinolates (7.6 µmol/g)
DISEASE REACTION: resistant to Blackleg (Leptosphaeria maculans asexual stage: Phoma lingam)

Origin & Breeding History: 'PA3CN164' is a male sterile line which contains the Ms8 gene construct in the heterozygous state. It was derived by backcrossing a doubled haploid line to a male sterile line used as the source of the bar and barnase genes. The doubled haploid line was backcrossed in Saskatoon, Saskatchewan, Canada in 2008. The final backcross was produced in 2012. 'PA3CN164' was selected in 2011 and 2012 on the basis of male sterility stability, expression of tolerance to glufosinate-ammonium herbicide and good combining ability with numerous restorer lines. Other selection parameters included height, vigour, maturity, blackleg resistance, oil content, fatty acid profile and glucosinolate content.

Tests & Trials: The comparative trials conducted during the summers of 2013 and 2014 in Saskatoon, Saskatchewan were set up in a RCB Design with 3 replications per variety. Each year, the plots consisted of 3 rows that were 6 metres in length with a spacing of 25 cm between rows and 50 cm between plots. The measured characteristics were based on 30 measurements of the cotyledon, leaf, flower and plant height and 60 measurements of the silique. The means were based on a two year average. Mean differences were significant at the 2% probability level based on LSD values.

Comparison tables for 'PA3CN164' with reference varieties 'PA0CN116', 'PPS01-140 A-Line' and '5440'

Cotyledon length (mm)

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean (LSD=2.1) 11.6 10.5 10.7 13.9
std. deviation 0.8 0.8 0.9 1.3

Cotyledon width (mm)

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean (LSD=2.7) 21.6 21.5 20.8 25.7
std. deviation 1.2 1.6 1.7 1.7

Leaf length (mm)

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean (LSD=11) 167 178 179 216
std. deviation 15 10 8 12

Petiole length (mm)

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean (LSD=6) 67 69 70 85
std. deviation 12 6 8 6

Days to flower

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean 43 45 42 40

Flower petal length (mm)

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean (LSD=0.7) 9.5 11.0 10.3 13.8
std. deviation 0.6 0.6 0.6 0.6

Flower petal width (mm)

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean (LSD=0.4) 4.6 5.5 5.3 6.0
std. deviation 0.5 0.5 0.5 0.6

Silique length (mm)

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean (LSD=2.5) 54.2 71.7 59.0 64.4
std. deviation 2.7 5.6 4.3 4.1

Beak length (mm)

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean (LSD=1.2) 10.0 13.0 8.2 11.9
std. deviation 0.7 1.0 1.1 0.9

Pedicel length (mm)

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean (LSD=1.6) 15.6 16.2 14.6 24.0
std. deviation 1.9 2.2 2.1 5.3

Days to maturity

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean 105 103 101 96

Plant height at maturity (cm)

  'PA3CN164' 'PA0CN116' 'PPS01-140 A-Line' '5440'
mean (LSD=3.9) 149 151 148 124
std. deviation 8 7 8 5

Click on image for larger view
Canola: 'PA3CN164' (far left) with reference varieties 'PPS01-140 A-Line' (centre left), 'PA0CN116' (centre right) and '5440' (far right)

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